O-7 A Biological Active Enquiry of Tradescantia zebrina (Wandering Jew/ Red Watergrass) on Blood Glucose Levels In Normal Sprague-Dawley Rats
Author(s):
S Newman , L Lindo , I Amarakoon
Type Of Study:
- Experimental or Intervention Study
- Quantitative
Year of Presentation:
2022
Objective: Phytochemical(s) extracted from Tradescantia
zebrina may significantly reduce blood glucose levels in
normal Sprague-Dawley rats when compared with respective control groups.
Methods: The stems and leaves of the plant were collected, air dried and milled. The plant material was then packed into a column and sequentially extracted using hexane, ethyl acetate and ethanol. The crude extracts obtained were bioassayed using normal Sprague-Dawley rats by intravenous administration using Oral Glucose Tolerance Test (OGTT). A phytochemical analysis was carried out according to a protocol by Roghini and Vijayalakshmi in 2018. An antioxidant inhibition assay was adapted and done as stated by Gul et al, 2017 using DPPH (2,2-dipheny l-1-picrylhydrazyl) as free radical.
Results: The crude extracts were found to contain multiple phytochemicals with the ethanol extract consisting of: Glycosides, Tannins, Flavonoids, Amino acids, Terpenoids and Coumarins. Both the ethanol and ethyl acetate extracts worked effectively as the positive control (ascorbic acidknown antioxidant scavenger) at the 200 ug/mL (p = 0.05). The OGTT revealed that at 50 mg/kg BW there is statistically significant difference (p = 0.05) between the ethanol extract and the control (DMSO) at the 120 (p = 0.002), 150 (p = 0.011) and 180 (p = 0.008) min.
Conclusion: The ethanol extract of Tradescantia zebrina illustrated hypoglycaemic effects in normal Sprague Dawley rats.