P-34 Antibacterial activity of Mustard leaves (Brassica juncea) – a potential for alternative antibiotic therapy?
Author(s):
B Ally-Charles, E Tyrell, A Koshilla, R Dukhie, T Persaud, L Ramsook, R Cecil, B Dey, M McKenzie
Type Of Study:
- Experimental or Intervention Study
Year of Presentation:
2023
Objective: To analyze the antibacterial activity of mustard leaves (Brassica juncea) against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and an Extended-spectrum β-lactamases (ESBL) K. pneumoniae.
Methods: The extracts from the leaves were obtained using three solvents; ethanol, hexane, and ethyl acetate and concentrated using a rotary evaporator. Serial dilution was used to obtain varying concentrations of the extracts (100- 0.78mg/ml) and sterile filter paper discs were placed in the extracts. The Kirby Bauer disc diffusion method was done using Mueller Hinton agar seeded with the five test bacteria. Discs were placed in triplicate on each plate. Discs soaked in pure solvent were used as the negative control and the antibiotics ciprofloxacin, ceftazidime and tetracycline constituted the positive controls. After incubation, any zone of inhibition around the discs was measured in millimeters and the results expressed as mean±Standard Deviation.
Results: Zones of inhibition were seen with extracts from ethyl acetate for 100mg/ml for K. pneumoniae (20.7 ± 5.0mm), K pneumoniae ESBL (13.0 ± 0.0mm), S. aureus (17.3 ± 6.8mm), E.coli (11.0 ± 1.0mm) and P. aeruginosa (13.3 ± 1.5mm). Zones for the 100mg/ml extracts against K. pneumoniae ESBL and P. aeruginosa were larger than the zones for tetracycline in some cases; and the zones for the ATCC K. pneumoniae were larger than those for both ceftazidime (14mm) and tetracycline (18mm).
Conclusion: B. juncea is potentially a novel alternative to antibiotic treatment and this study provides a template for investigation of its antifungal and phytochemical properties.